成人在线视频国产,伊人伊狠亚洲综合影院,亚洲国产天堂久久综合,欧美国产日韩综合

題目: Single-cell transcriptomes and runx2b?/? mutants reveal the genetic signatures of intermuscular bone formation in zebrafish

作者:  Chun-Hong Nie, Shi-Ming Wan, Yu-Long Chen, Ann Huysseune, Ya-Ming Wu, JiaJia Zhou, Alexandre Wagner Silva Hilsdorf, Wei-min Wang, Paul Eckhard Witten, QiangLin, Ze-Xia Gao

刊物: National science review

年卷期頁(yè): 2022

摘要：Intermuscular bones (IBs) are mineralized spicules, present in the myosepta of many, but not all teleost species. IBs are often small and sharp, and they consequently limit how the fish can be processed, cause injury or trauma if lodged in consumers' throat or mouth and therefore affect the appeal of the fish to many consumers. The development of IBs in teleosts is still not fully understood and the molecular basis of IB development remains to be established. Here, the characteristics of IB tissue is evaluated based on single-cell transcriptomic in wild-type zebrafish. The analysis defined 18 distinct cell types. Differentiation trajectories showed that IBs are derived from tendons and that a core tendon-osteoblast cell lineage is related to IB formation. In particular, the functions of 10 candidate genes were evaluated via CRISPR-Cas9 mutants. Among those, runx2b?/? mutants completely lost IBs, while swimming performance, growth, and bone mineral density were not significantly different form runx2b+/+zebrafish. Comparative scRNA-seq analysis in runx2b?/?and runx2b+/+zebrafish revealed the role of osteoblasts in IB formation. In addition, differentially expressed genes were enriched in TGF-β/BMP pathway after runx2b deletion. This study provide evidence for a crucial role of runx2b regulation in IB formation. Genetic breeding can target runx2b regulation and generate strains of commercial fish species without IBs, which can improve the safe consumption and economic value of many farmed fish species.